- About EHSC
- Zebrafish Biomedical Research
- Chemical Exposure
- Career Development
- Community Engagement
- Integrated Health
- Pilot Projects
Zebrafish are photoperiodic with respect to their breeding. They will produce embryos every morning shortly after sunrise. Depending on the demand for embryos, an equal number of males and females are selected for production on the previous day.
Tanks are equipped with green netting substrate in raised baskets to initiate spawning. Controlled light cycle (14-hours light/10 hours dark) is utilized to mimic photoperiods.
Spawning will occur shortly after lights come on in the morning. Eggs are then collected, cleaned, screened and transferred to the incubator in the Lab, or utilized for future stock.
The liquid handler is utilized at the SARL lab to increased efficiency and accuracy for loading chemicals into 96 well plates. This machine has a computer controlled robotic 96 well format head that holds pipette tips for extracting chemical stock and serial diluting to various regions of each plate.
Early Developmental Behavior
Single zebrafish larvae are placed in each well of a 96-well plate allowing the simultaneous monitoring of larvae using an automated video-tracking system. Responses of each larva to 20 minutes light ON, 10 minutes lights OFF, and then lights ON for 5 minutes are recorded and quantified. Normal control larvae responses are documented and compared to 5 dpf (days post fertilization) chemically exposed embryo assays. We currently have 4 behavior boxes in use at SARL.
The dechorionator provides a means of automating the dechorionating process. Embryos are dechorionated at 4hpf (hours post fertilization). 4,800 embryos can be processed at one time.