This Core provides equipment, services, and consulting expertise related to the detection of phenotypic and morphologic changes at the tissue, cell, and subcellular levels. State-of-the-art techniques in histopathology immunohistology, light and electron microscopy, and image analysis combine to offer the EHS Center investigators the highest quality analytical tools, diagnostic interpretations, and consulting expertise for evaluation of mechanisms of toxicant action and carcinogenesis.
The Histopathology Laboratory, located in the College of Veterinary Medicine, is well equipped with all the necessary instruments for histological procedures utilizing paraffin- and plastic-embedded tissues and frozen tissues: The laboratory has all the necessary instruments to complete these tasks. Significant equipment includes: microtomes, a Tissue-Tek VIP tissue processor, a Tissue-Tek tissue-embedding center, automatic slide stainer, cryostat, and a Jung polycut E microtome used for large plastic blocks and very dense specimens.
A new and exciting addition to the equipment of the Histopathology Laboratory is a Dako automated immunohistochemical stainer that permits high throughput for the ever increasing number of immunohistochemical analyses. This equipment allows for the review of glass slides of tissues or cell cultures that have been routinely stained with hematoxylin-eosin, a variety of special histochemical stains, e.g., iron, glycogen, immunohistochemical staining or staining by in situ hybridization techniques. The Electron Microscopy laboratory is reasonably equipped to prepare tissues for most procedures requiring electron microscopy. This laboratory has an ultramicrotome (Sorvall MT5000) and a JB4 microtome for thick sections.
Ms. Kay Fischer, a Senior Faculty Research Assistant in the College of Veterinary Medicine, provides technical assistance in histology, image analysis, and electron microscopy. Ms. Fischer has a M.S. degree in Biology from Stephen F. Austin State University, Nacogdoches, Texas, and has been associated with the Core since 1995. Previously she was employed by the College of Veterinary Medicine, Texas A&M University in the Image Analysis Laboratory, where she gained valuable experience in the techniques involving morphologic evaluation of tissues. Ms. Fischer is highly accomplished in preparing tissue for light and transmission (TEM) and scanning electron microscopy (SEM). As an operator of these respective microscopes, she possesses excellent photographic skills, and is well-versed in immunohistochemistry and in situ hybridization techniques.