Pacific Northwest Center for Translational Environmental Health Research

1. CUSTOMER REGISTRATION

Those who wish to place an order or establish Oregon State ZBR as a supplier must complete the customer registration. After successfully completing the registration form, OSU supplier information, W9 and how to set up payment will be provided.

Customer registration form

_{*for international orders, please contact a core director after registration.}

2. PLACE ORDER FOR FISH OR SERVICES

As a service to the community and EHS members, we offer the ability to order specific pathogen free fish (SPF) for two wildtype line and toxicity testing in embryonic and adult zebrafish. There are discounts available to those depending on their affiliation to the ZBR. The order form applies to both OSU and external members. An order form is required to receive our services.

FISH

The SPF monitored AB and 5D Tropical lines were originally screened for Pseudoloma neurophilia using qRTPCR (Sanders, 2011). After entering the SPF facility all lines are routinely screened for Pseudoloma neurophilia and other pathogens of concern (Kent, 2011).Since their introduction into the SARL facility, the lines have been maintained using a method that is in accordance with the ZIRC's protocol for AB and wild type next generation propagation. Each new generation is derived from the offspring of no less than 25 small group crosses, containing 3 males and up to 3 females each. Equal numbers of contributors are pulled from each cross to create the next generation. Embryos are screened daily until 5 dpf for the morphological defect.

• Sanders JL, Kent ML (2011) Development of a sensitive assay for the detection of Pseudoloma neurophilia in laboratory ¬populations of the zebrafish Danio rerio. Dis Aquat Org 96:145-156
• Kent ML, Buchner C, Watral VG, Sanders JL, LaDu J, Peterson TS, Tanguay RL (2011) Development and maintenance of a specific pathogen-free (SPF) zebrafish research facility for Pseudoloma neurophilia. Dis Aquat Org 95:73-79

There are two types of fish order – self collection (ie: local pick up) or delivery (only within the United States). All delivery orders include the cost of shipping, supplies and handling.

Embryos are bleached at 8 hours post fertilization (hpf) and will be approximately 48 hours upon arrival. Delivery orders must be received with at least 2 weeks lead time before desired receipt date. Shipments are sent only on Tuesday and Wednesday. Self collection orders must be placed 72 hours before needed.

SERVICES

Zebrafish developmental toxicity testing is available to all those who wish to assess their samples. Additionally, we offer adult and larval behavior assays.

Fish order form

Services order form

3. ORDER APPROVAL

After placing an order, it will take 24-48 business hours to be approved. If you do not receive an order approval within this time frame, contact [email protected]. We will communicate if anything about the order needs to be adjusted.

4. ORDER COMPLETE

Shipments are sent only on Tuesday and Wednesday. A Fedex notification will be sent when shipped. Self collection orders must be For services, a report will be provided.

5. INVOICE FOR ORDER

Once shipment and/or services are rendered, an invoice will be sent. OSU only accepts checks. Payments are NET30.