All fish are Specific Pathogen Free (SPF) for Pseudoloma neurophilia.
The SPF monitored AB and 5D Tropical lines were originally screened for Pseudoloma neurophilia using qRTPCR (Sanders, 2011). After entering the SPF facility all lines are routinely screened for Pseudoloma neurophilia and other pathogens of concern (Kent, 2011).
Since their introduction into the SARL facility, the lines have been maintained using a method that is in accordance with the ZIRC's protocol for AB and wild type next generation propagation. Each new generation is derived from the offspring of no less than 25 small group crosses, containing 3 males and up to 3 females each. Equal numbers of contributors are pulled from each cross to create the next generation. Embryos are screened daily until 5 dpf for morphological defect.
Sanders JL, Kent ML (2011) Development of a sensitive assay for the detection of Pseudoloma neurophilia in laboratory ¬populations of the zebrafish Danio rerio. Dis Aquat Org 96:145-156
Kent ML, Buchner C, Watral VG, Sanders JL, LaDu J, Peterson TS, Tanguay RL (2011) Development and maintenance of a specific pathogen-free (SPF) zebrafish research facility for Pseudoloma neurophilia. Dis Aquat Org 95:73-79
Please review the order process prior to using the on-line form below.
To place an order, please fill out the request form below.You will be contacted regarding the availability of your request.An estimate will be issued for your approval.Once the estimate has been approved, your order will be scheduled and shipped.An invoice will be included with your fish.Please remit payment upon receipt of the order.
ITEM ACADEMIC/NON-COMMERCIAL COMMERCIAL
AB, Adult Pair $150.00 $300.00
5D, Adult Pair $150.00 $300.00
AB, Embryos (100) $150.00 $300.00
5D, Embryos (100) $150.00 $300.00
Shipping charges are to be paid by the recipient. We ship via Fedex and World Courier. Please contact us for questions regarding shipping costs.
||Depending on the size of the order, a $35.00-$45.00 charge is applied per box. Due to a number of factors, including order size and temperature requirements, multiple boxes may be used to ship your order. Heat packs are used if necessary, and are included in the box fee.
|International Paperwork Fee
||Due to import/export paperwork requirements, all international orders will be assessed a $150.00 paperwork fee.